Fk2 antibody
WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono … WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be …
Fk2 antibody
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WebJan 7, 2024 · The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterized by one-dimensional Western blotting. The immunoglobulin … WebImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT.
WebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... WebAnti-Ubiquitinylated proteins Antibody, clone FK1 04-262 Sigma-Aldrich Anti-Ubiquitinylated proteins Antibody, clone FK1 Download Zoom Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against …
WebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking. WebImmunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ …
WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight.
WebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1 , 2 WARNING This … fly by tokyo machineWebMay 15, 2005 · Separation of ubiquitinated Syk using an antiubiquitin antibody. Washed platelets were activated with convulxin (100 ng/mL) for 15 seconds. Cells were lysed with RIPA buffer and treated with FK2 antibody for 1 hour at 4°C, followed by Protein A/G PLUS-agarose for an additional hour. Supernatants were treated with anti-Syk using the … greenhouses in amarillo txWebSep 13, 2024 · The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. … greenhouses in brantford ontarioWeb11 rows · Anti-Ubiquitinylated proteins Antibody, clone FK2. Background Information. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a … fly by the seat of your pants 意味Web(B and C) Mono- and poly-ubiquitinated proteins detection using the FK2 specific monoclonal antibody (B) Quantification of ubiquitinated protein detection after normalization to Actin (C). (D) Control and treated RHEs were stained with haematoxylin–eosin (n = 2, 1 independent bank). Bar = 10 μm. flyby tours egyptWebThis Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting … fly by to goWebMouse Monoclonal Antibody Shop MilliporeSigma™ anti-Ubiquitin Clone: FK2, at Fishersci.com. Fisher Scientific ; ... All Primary Antibodies ST1200100UG Print … fly by to go desert ridge