Mouse hepatocyte isolation
Nettet1. jan. 2013 · Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties … NettetHere we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital. References
Mouse hepatocyte isolation
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Nettet22. nov. 2011 · Hepatocyte isolation, by the method described herein, was highly reproducible as demonstrated by the generation on average of 18–20 million cells … Nettet17. des. 2024 · A frequently observed outcome from a hepatocyte isolation from different Percoll fractions is shown in the graph expressed as viable/dead cells from each …
NettetPrimary mouse hepatocytes are an important tool in the biomedical research field for the assessment of hepatocyte function. Several methods for hepatocyte isolation … Nettet1. aug. 2024 · The method of primary mouse hepatocyte isolation was modified from the classic two-step collagenase perfusion technique [67]. The specific steps were as …
Nettet1. jan. 2024 · However, isolation of mouse liver cells is challenging and requires specific technical procedure and skills. Here, we elaborate a method for effective isolation of both primary hepatocytes and KC from adult liver of the same mouse. This protocol can be used for isolation of liver cells from both wild-type (WT) and genetically-engineered mice. Nettet7 timer siden · TOTUM-070 is a patented polyphenol-rich blend of five different plant extracts showing separately a latent effect on lipid metabolism and potential synergistic properties. In this study, we investigated the health benefit of such a formula. Using a preclinical model of high fat diet, TOTUM-070 (3 g/kg of body weight) limited the HFD …
NettetIsolated hepatocytes and KC serve as important tools to study mechanistic events during NASH in an in vitro setting. Because mice and humans share identical genes, …
Nettet2 dager siden · Nevertheless, unlike liver fat, liver inflammation and hepatocyte ballooning injury remained statistically significantly higher in Tet2 −/−-transplanted mice compared to wild-type controls ... excel lock sheet positionNettetPrimary mouse hepatocytes are an important tool in the biomedical research field for the assessment of hepatocyte function. Several methods for hepatocyte isolation have been published; however, many of these methods require extensive handling and can therefore compromise the viability and function of the isolated cells. excel lock text box locationNettetHere we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital. MeSH terms Animals bs4 tooltipNettetIsolation and culture of adult mouse hepatocytes. The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, … bs4 to bs32NettetCD73 (ecto-5′-nucleotidase) hepatocyte levels differ across mouse strains and contribute to Mallory-Denk body formation. Hepatology … excel lock text boxNettetIt has been optimized for a high yield of non-parenchymal mouse liver cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells, while preserving most cell surface epitopes. Dissociated cells can be cultured or isolated using MACS ® Technology. Detailed product information excel lock specific cells on worksheetNettet26. mar. 2013 · Along with the improvement in enzyme digested hepatocyte isolation techniques in recent years, hepatocyte culture has been researched extensively [1-3]. Numerous culture methods have been used or are under investigation to facilitate the functional cell growth of primary hepatocytes in vitro . bs4 to bs5