WebThe Immun-Star WesternC chemiluminescence kit is designed for use with Precision Plus Protein™ WesternC™ standards and CCD imaging systems. It offers femtogram-level … WebHome Western Blotting Immunodetection Using BCIP/NBT Substrate Immunodetection Using BCIP/NBT Substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue …
Western Blot Detection of Phosphorylation Events - Bio-Rad
WebJan 25, 1987 · We have examined the effects of the "differentiating agent," sodium butyrate, on the induction of alkaline phosphatase in human colonic tumor cell line LS174T. Culture of these cells in the presence of 2 mM butyrate caused this activity to increase from less than 0.0001 unit/mg of protein to greater … WebThe new Chromogenic Western Blot Kit allows for quick, visual reading of Western blots in unpurified cell extract. The kit can be used to identify primary mouse antibodies on a Western blot using immunodetection. It contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents. Chemiluminescence Detection date ideas little rock ar
Mitogen-activated protein kinase phosphatase-3 (MKP-3) in the …
WebAnti-Mouse IgA (α-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA [ 4] [ 5]. The product can also be used for western blot (1:30,000). Packaging 0.25, 0.5, 1 mL Physical form Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl 2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide. Disclaimer WebJan 1, 2024 · Western Blotting Using Alkaline Phosphatase-Labeled Secondary Jan 01, 2024 Solutions PBS – sodium phosphate-buffered (100 mM; pH 7.2) isotonic (0.9% NaCl, w/v) … WebWestern blotting is typically performed by probing the blocked membrane with a primary antibody that recognizes a specific protein or epitope on a group of proteins (e.g., SH2 domain or phosphorylated tyrosine). biweekly budget template for excel