Phospho eif2 alpha antibody

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August undefined, 2024

WebPhospho-EIF2S1 (Ser51) antibody for WB, ELISA and reacts with Human, mouse, rat. ... eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51 ... EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis ... WebGuarantees. Rabbit polyclonal antibody to eIF2 alpha (phospho Ser51). This antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51. Synthetic …

Phospho-GCN2 (Thr898) Antibody Cell Signaling …

WebAnti-EIF2 α Antibody detects endogenous levels of total EIF2 α protein. Immunogen The antiserum was produced against synthesized peptide derived from human eIF2 alpha. Immunogen Range: 21-70 Features and Benefits Evaluate … WebSpecificity. This antibody reacts to human eIF2 alpha/EIF2S1 (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF2 alpha/EIF2S1 without phosphorylation at Ser51. This antibody may also react to mouse or rat Phospho-eIF2 alpha/EIF2S1 (Ser51). Isotype. highland organic market

Anti-eIF2α Antibody (D-3) SCBT - Santa Cruz Biotechnology

WebPhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. Formulation： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source： Polyclonal, Rabbit,IgG Dilution： IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … WebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … highland orchards downingtown pa fire

The structural basis of translational control by eIF2 ... - Nature

(9721) Phospho-eIF2α (Ser51) Antibody - CiteAb

WebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2) WebMay 13, 2024 · For eIF2 phosphorylation, purified PKR and eIF2 (0.08 µg PKR per 10 µg of eIF2) were mixed at room temperature in eIF2 storage buffer supplemented with 0.5 mM ATP, 10 mM MgCl 2 and 5 mM NaF, and ... highland orchards wilmington delawareWebAntibodies are purified by protein A and peptide affinity chromatography. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism of downregulating protein … how is hydrostatic pressure measured

"WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) suitable for the … " - Phospho eif2 alpha antibody

Phospho eif2 alpha antibody

Phospho-eIF2α (Ser51) Antibody Cell Signaling Technology

WebSubunit Structure: Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. … WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ...

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WebProduct Name eIF2 alpha (Phospho-Ser51) Rabbit mAb; Host Species Rabbit; Clonality Monoclonal; Isotype Rabbit IgG; Purification Affinity-chromatography; Applications WB … WebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated …

WebPhospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize … WebPhosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important …

WebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm … WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of …

WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ...

http://immunoway.com/Home/22/YP0093 highland orchards mobile home park pa highland ornate door ffxivWebEIF2 alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody; ... All lanes : Anti-EIF2S1 (phospho S51) antibody (ab131505) at 1/500 dilution Lane 1 : K562 cell extract Lane 2 : K562 cell extract treated with IFN alpha highland orchards farmWebBinding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). highland organic restaurantWebYour search returned 47 eIF2 alpha, phospho Antibodies across 9 suppliers. Showing 9 of 9 suppliers (47 products total) > >> Select All. Select up to 5 products from below to compare or request more information. Request Info for all … highland ornate ltd. t/a ornate healthcareWebWestern Blot. Detection of Human Phospho-eIF2 alpha (S52) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 … how is hyperglycemia treatedWebIHC-P analysis of human breast carcinoma tissue using GTX50300 eIF2 alpha (phospho Ser51) antibody. GTX50300 WB Image WB analysis of K562 cells untreated or treated with IFN-α (100ng/ml, 20min) lysates using GTX50300 eIF2 alpha (phospho Ser51) antibody (Lane 3 and 4) and eIF2α antibody (Lane 1 and 2). ... how is hydropower used to create energy