Pinene synthase

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August undefined, 2024

WebDec 12, 2024 · Based on previous studies, most of the monoterpenoid synthases are multifunctional synthases. (-)-α-pinene synthase can produce α-pinene, β-pinene, β-phellandrene, β-myrcene, and camphene, and (-)-limonene synthase can produce α-pinene, β-pinene, and β-myrcene (Keeling et al. 2011; Martin et al. 2004). WebDescription. Cyclase II of Salvia officinalis (sage) gives about equal parts (-)-α-pinene, (-)-β-pinene and (-)-camphene, plus traces of other monoterpenoids. (3S)-Linalyl diphosphate …

alpha-pinene synthase - Wikipedia

(–)-α-Pinene synthase (EC 4.2.3.119, (–)-α-pinene/(–)-camphene synthase, (–)-α-pinene cyclase) is an enzyme with systematic name geranyl-diphosphate diphosphate-lyase [cyclizing, (–)-α-pinene-forming]. This enzyme catalyses the following chemical reaction geranyl diphosphate (–)-α-pinene + diphosphate Cyclase II of Salvia officinalis (sage) gives about equal parts (–)-α-pinene, (–)-β-pinene and (–)-c… WebHere, we provide an overview on the synthesis and metabolic pathways, regulatory pathways, and key enzymes in the biosynthetic pathways of terpenoids. This paper will … recipe for easy lemon meringue pie

Bacterial Production of Pinene by a Laboratory-Evolved Pinene …

WebSep 12, 2024 · The C. sativa β-pinene synthase is as yet unidentified, but it is common for α-and β-pinene synthases in other species to produce some amount of β-myrcene as a by-product [ 20 ]. Cis-ocimene is produced as a single product by TPS13 [ 7 ], and shows no correlation to other terpenes ( Fig 3 ). WebMay 31, 2016 · Pinene synthase tPt1 was expressed in the constructed engineered yeast, and the titer of pinene reached 0.166 mg/L. GPP is the direct precursor of pinene, … WebMay 17, 2016 · In this context, the objectives of present study were to identify, isolate, and functionally characterize α-pinene synthase genes from P. pinaster and P. pinea using … unlucky baseball out

Improved pinene production in a recombinant yeast by fusion

Monoterpene Synthases from Grand Fir (Abies grandis)

WebAug 15, 2024 · We used the pipeline to screen pinene synthase variant libraries, with mutations in three areas of plasticity, capable of producing multiple monoterpene products. WebPinene synthase. In enzymology, a pinene synthase ( EC 4.2.3.14) is an enzyme that catalyzes the chemical reaction. Hence, this enzyme has one substrate, geranyl … recipe for easy minestrone soupWebAug 30, 2024 · Pinene is an active natural monoterpene from plants and has important applications in flavorings, fragrances,and pesticides. Especially, pinene dimers are regarded as renewable fuels with high density. However, the microbial pinene production was limited by the low activity pinene synthase. In this study, the pinene synthase activity was … recipe for easy moist brownies

"WebNational Center for Biotechnology Information " - Pinene synthase

Pinene synthase

Two terpene synthases in resistant - Wiley Online Library

WebApr 6, 2016 · In vitro enzyme assay showed that, in contrast to most monoterpene synthases, PlPIN encoded an α-pinene synthase which converted GPP into α-pinene … WebAug 24, 2024 · A pinene synthase from A. grandis that produces both (−)-α- and (−)-β-pinene in pH 7.5 assay buffer (Bohlmann et al., 1997). In our experiments, the neutral conditions of pH 7 may contribute to (+)-/(−)-pinenes. The results of enzyme activity are consistent with those detected in volatile oil from leaves.

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WebMay 17, 2024 · Pinene is generally synthesized in plants, converted from geranyl diphosphate (GPP) by pinene synthase (PS), while GPP is synthesized by geranyl … WebAug 29, 1997 · Pinene synthase has previously received considerable attention as a major defense-related monoterpene synthase in conifers (18, 19). In the grand fir cDNA library, which was synthesized from mRNA obtained from wound-induced sapling stems, clones corresponding to pinene synthase are at least 10 times more abundant than clones for …

Web(+)-α-pinene synthase (EC 4.2.3.121, (+)-α-pinene cyclase, cyclase I) is an enzyme with systematic name geranyl-diphosphate diphosphate-lyase [cyclizing, (+)-α-pinene-forming]. [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction geranyl diphosphate (+)- α-pinene + diphosphate WebCrystal structural data for (4S)-limonene synthase [(4S)-LS] of spearmint (Mentha spicata L.) were used to infer which amino acid residues are in close proximity to the substrate and carbocation intermediates of the enzymatic reaction. Alanine-scanning mutagenesis of 48 amino acids combined with enzyme fidelity analysis [percentage of (−)-limonene …

WebNCBI. proteins. (–)-β-Pinene synthase (EC 4.2.3.120, β-geraniolene synthase, (–)- (1S,5S)-pinene synthase, geranyldiphosphate diphosphate lyase (pinene forming)) is an …

WebHere, we characterized two terpene synthase genes, α-pinene synthase (PmTPS4) and longifolene synthase (PmTPS21), identified in resistant P. massoniana and investigate …

Web(+)-α-pinene synthase (EC 4.2.3.121, (+)-α-pinene cyclase, cyclase I) is an enzyme with systematic name geranyl-diphosphate diphosphate-lyase [cyclizing, (+)-α-pinene … recipe for easy muffinsWebMay 31, 2024 · The fluidities and permeabilities of cell membranes of Candida glycerinogenes decrease as pinene levels increase resulting in adverse effects on cell growth, and the biomass of yeast cells cultivated in shake flask systems are improved by exogenous addition of trehalose and ergosterol. Pinene is a commercially important … unlucky bird lightojWebsynthase, (2)-limonene synthase, and a pinene synthase that produces both (2)-a- and (2)-b-pinene (Fig. 1). This is the first report of the isolation of any cDNA encoding a … recipe for easy overnight baked french toastWebMay 1, 2004 · cDNA isolation, functional expression, and characterization of (+)-α-pinene synthase and (−)-α-pinene synthase from loblolly pine Pinus taeda: stereocontrol in pinene biosynthesis. Archives of Biochemistry and Biophysics, 411 (2003), pp. 267-276. View PDF View article View in Scopus Google Scholar. recipe for easy mac and cheese 3 ingredientsWebAug 2, 2024 · Terpenoid synthase (TPS), located at the branch point of the isoprenoid pathway, catalyzes GPP to form the monoterpenoid skeleton directly. Therefore, TPS is not only the key enzyme for terpenoid synthesis but also the main inducer for the structural diversity of terpenoids ( Chen et al., 2011 ). recipe for easy pecan pie cheesecakeWebMar 18, 2024 · Pinene (C10H16) is a monoterpenoid compound with a molecular weight of 136.23 Da. According to its features of noncytotoxicity and tastelessness, pinene has been increasingly used in modern industries, such as rubber, coatings, printing, food packaging, and hygiene [ 1, 2 ]. unlucky break for a competitor crossword clueWebS-limonene synthase is a model monoterpene synthase that cyclizes geranyl pyrophosphate (GPP) to form S-limonene. It is a relatively specific enzyme as the … unlucky british folksinger crossword